>NM_000277.1 Homo sapiens phenylalanine hydroxylase (PAH), mRNA
CAGCTGGGGGTAAGGGGGGCGGATTATTCATATAATTGTTATACCAGACGGTCGCAGGCTTAGTCCAATT
GCAGAGAACTCGCTTCCCAGGCTTCTGAGAGTCCCGGAAGTGCCTAAACCTGTCTAATCGACGGGGCTTG
GGTGGCCCGTCGCTCCCTGGCTTCTTCCCTTTACCCAGGGCGGGCAGCGAAGTGGTGCCTCCTGCGTCCC
CCACACCCTCCCTCAGCCCCTCCCCTCCGGCCCGTCCTGGGCAGGTGACCTGGAGCATCCGGCAGGCTGC
CCTGGCCTCCTGCGTCAGGACAAGCCCACGAGGGGCGTTACTGTGCGGAGATGCACCACGCAAGAGACAC
CCTTTGTAACTCTCTTCTCCTCCCTAGTGCGAGGTTAAAACCTTCAGCCCCACGTGCTGTTTGCAAACCT
GCCTGTACCTGAGGCCCTAAAAAGCCAGAGACCTCACTCCCGGGGAGCCAGCATGTCCACTGCGGTCCTG
GAAAACCCAGGCTTGGGCAGGAAACTCTCTGACTTTGGACAGGAAACAAGCTATATTGAAGACAACTGCA
ATCAAAATGGTGCCATATCACTGATCTTCTCACTCAAAGAAGAAGTTGGTGCATTGGCCAAAGTATTGCG
CTTATTTGAGGAGAATGATGTAAACCTGACCCACATTGAATCTAGACCTTCTCGTTTAAAGAAAGATGAG
TATGAATTTTTCACCCATTTGGATAAACGTAGCCTGCCTGCTCTGACAAACATCATCAAGATCTTGAGGC
ATGACATTGGTGCCACTGTCCATGAGCTTTCACGAGATAAGAAGAAAGACACAGTGCCCTGGTTCCCAAG
AACCATTCAAGAGCTGGACAGATTTGCCAATCAGATTCTCAGCTATGGAGCGGAACTGGATGCTGACCAC
CCTGGTTTTAAAGATCCTGTGTACCGTGCAAGACGGAAGCAGTTTGCTGACATTGCCTACAACTACCGCC
ATGGGCAGCCCATCCCTCGAGTGGAATACATGGAGGAAGAAAAGAAAACATGGGGCACAGTGTTCAAGAC
TCTGAAGTCCTTGTATAAAACCCATGCTTGCTATGAGTACAATCACATTTTTCCACTTCTTGAAAAGTAC
TGTGGCTTCCATGAAGATAACATTCCCCAGCTGGAAGACGTTTCTCAATTCCTGCAGACTTGCACTGGTT
TCCGCCTCCGACCTGTGGCTGGCCTGCTTTCCTCTCGGGATTTCTTGGGTGGCCTGGCCTTCCGAGTCTT
CCACTGCACACAGTACATCAGACATGGATCCAAGCCCATGTATACCCCCGAACCTGACATCTGCCATGAG
CTGTTGGGACATGTGCCCTTGTTTTCAGATCGCAGCTTTGCCCAGTTTTCCCAGGAAATTGGCCTTGCCT
CTCTGGGTGCACCTGATGAATACATTGAAAAGCTCGCCACAATTTACTGGTTTACTGTGGAGTTTGGGCT
CTGCAAACAAGGAGACTCCATAAAGGCATATGGTGCTGGGCTCCTGTCATCCTTTGGTGAATTACAGTAC
TGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCA
CGGAGTTCCAGCCCCTGTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGGAACTTTGC
TGCCACAATACCTCGGCCCTTCTCAGTTCGCTACGACCCATACACCCAAAGGATTGAGGTCTTGGACAAT
ACCCAGCAGCTTAAGATTTTGGCTGATTCCATTAACAGTGAAATTGGAATCCTTTGCAGTGCCCTCCAGA
AAATAAAGTAAAGCCATGGACAGAATGTGGTCTGTCAGCTGTGAATCTGTTGATGGAGATCCAACTATTT
CTTTCATCAGAAAAAGTCCGAAAAGCAAACCTTAATTTGAAATAACAGCCTTAAATCCTTTACAAGATGG
AGAAACAACAAATAAGTCAAAATAATCTGAAATGACAGGATATGAGTACATACTCAAGAGCATAATGGTA
AATCTTTTGGGGTCATCTTTGATTTAGAGATGATAATCCCATACTCTCAATTGAGTTAAATCAGTAATCT
GTCGCATTTCATCAAGATTAATTAAAATTTGGGACCTGCTTCATTCAAGCTTCATATATGCTTTGCAGAG
AACTCATAAAGGAGCATATAAGGCTAAATGTAAAACACAAGACTGTCATTAGAATTGAATTATTGGGCTT
AATATAAATCGTAACCTATGAAGTTTATTTTCTATTTTAGTTAACTATGATTCCAATTACTACTTTGTTA
TTGTACCTAAGTAAATTTTCTTTAGGTCAGAAGCCCATTAAAATAGTTACAAGCATTGAACTTCTTTAGT
ATTATATTAATATAAAAACATTTTTGTATGTTTTATTGTAATCATAAATACTGCTGTATAAGGTAATAAA
ACTCTGCACCTAATCCCCATAACTTCCAGTATCATTTTCCAATTAATTATCAAGTCTGTTTTGGGAAACA
CTTTGAGGACATTTATGATGCAGCAGATGTTGACTAAAGGCTTGGTTGGTAGATATTCAGGAAATGTTCA
CTGAATAAATAAGTAAATACATTATTGAAAAGCAAATCTGTATAAATGTGAAATTTTTATTTGTATTAGT
AATAAAACATTAGTAGTTTA
>PAH mutant
CAGCTGGGGGTAAGGGGGGCGGATTATTCATATAATTGTTATACCAGACGGTCGC AGGCTTAGTCCAATTGCAGAGAACTCGCTTCCCAGGCTTCTGAGAGTCCCGGAAG TGCCTAAACCTGTCTAATCGACGGGGCTTGGGTGGCCCGTCGCTCCCTGGCTTCT TCCCTTTACCCAGGGCGGGCAGCGAAGTGGTGCCTCCTGCGTCCCCCACACCCTC CCTCAGCCCCTCCCCTCCGGCCCGTCCTGGGCAGGTGACCTGGAGCATCCGGCAG GCTGCCCTGGCCTCCTGCGTCAGGACAAGCCCACGAGGGGCGTTACTGTGCGGAG ATGCACCACGCAAGAGACACCCTTTGTAACTCTCTTCTCCTCCCTAGTGCGAGGT TAAAACCTTCAGCCCCACGTGCTGTTTGCAAACCTGCCTGTACCTGAGGCCCTAA AAAGCCAGAGACCTCACTCCCGGGGAGCCAGCATGTCCACTGCGGTCCTGGAAAA CCCAGGCTTGGGCAGGAAACTCTCTGACTTTGGACAGGAAACAAGCTATATTGAA GACAACTGCAATCAAAATGGTGCCATATCACTGATCTTCTCACTCAAAGAAGAAG TTGGTGCATTGGCCAAAGTATTGCGCTTATTTGAGGAGAATGATGTAAACCTGAC CCACATTGAATCTAGACCTTCTCGTTTAAAGAAAGATGAGTATGAATTTTTCACC CATTTGGATAAACGTAGCCTGCCTGCTCTGACAAACATCATCAAGATCTTGAGGC ATGACATTGGTGCCACTGTCCATGAGCTTTCACGAGATAAGAAGAAAGACACAGT GCCCTGGTTCCCAAGAACCATTCAAGAGCTGGACAGATTTGCCAATCAGATTCTC AGCTATGGAGCGGAACTGGATGCTGACCACCCTGGTTTTAAAGATCCTGTGTACC GTGCAAGACGGAAGCAGTTTGCTGACATTGCCTACAACTACCGCCATGGGCAGCC CATCCCTCGAGTGGAATACATGGAGGAAGAAAAGAAAACATGGGGCACAGTGTTC
AAGACTCTGAAGTCCTTGTATAAAACCCATGCTTGCTATGAGTACAATCACATTT TTCCACTTCTTGAAAAGTACTGTGGCTTCCATGAAGATAACATTCCCCAGCTGGA AGACGTTTCTCAATTCCTGCAGACTTGCACTGGTTTCCGCCTCCGACCTGTGGCT GGCCTGCTTTCCTCTCGGGATTTCTTGGGTGGCCTGGCCTTCCGAGTCTTCCACT GCACACAGTACATCAGACATGGATCCAAGCCCATGTATACCCCCAAACCTGACAT CTGCCATGAGCTGTTGGGACATGTGCCCTTGTTTTCAGATCGCAGCTTTGCCCAG TTTTCCCAGGAAATTGGCCTTGCCTCTCTGGGTGCACCTGATGAATACATTGAAA AGCTCGCCACAATTTACTGGTTTACTGTGGAGTTTGGGCTCTGCAAACAAGGAGA CTCCATAAAGGCATATGGTGCTGGGCTCCTGTCATCCTTTGGTGAATTACAGTAC TGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCC AAAATTACACTGTCACGGAGTTCCAGCCCCTGTATTACGTGGCAGAGAGTTTTAA TGATGCCAAGGAGAAAGTAAGGAACTTTGCTGCCACAATACCTCGGCCCTTCTCA GTTCGCTACGACCCATACACCCAAAGGATTGAGGTCTTGGACAATACCCAGCAGC TTAAGATTTTGGCTGATTCCATTAACAGTGAAATTGGAATCCTTTGCAGTGCCCT CCAGAAAATAAAGTAAAGCCATGGACAGAATGTGGTCTGTCAGCTGTGAATCTGT TGATGGAGATCCAACTATTTCTTTCATCAGAAAAAGTCCGAAAAGCAAACCTTAA TTTGAAATAACAGCCTTAAATCCTTTACAAGATGGAGAAACAACAAATAAGTCAA AATAATCTGAAATGACAGGATATGAGTACATACTCAAGAGCATAATGGTAAATCT TTTGGGGTCATCTTTGATTTAGAGATGATAATCCCATACTCTCAATTGAGTTAAA TCAGTAATCTGTCGCATTTCATCAAGATTAATTAAAATTTGGGACCTGCTTCATT CAAGCTTCATATATGCTTTGCAGAGAACTCATAAAGGAGCATATAAGGCTAAATG TAAAACACAAGACTGTCATTAGAATTGAATTATTGGGCTTAATATAAATCGTAAC CTATGAAGTTTATTTTCTATTTTAGTTAACTATGATTCCAATTACTACTTTGTTA TTGTACCTAAGTAAATTTTCTTTAGGTCAGAAGCCCATTAAAATAGTTACAAGCA TTGAACTTCTTTAGTATTATATTAATATAAAAACATTTTTGTATGTTTTATTGTA ATCATAAATACTGCTGTATAAGGTAATAAAACTCTGCACCTAATCCCCATAACTT CCAGTATCATTTTCCAATTAATTATCAAGTCTGTTTTGGGAAACACTTTGAGGAC ATTTATGATGCAGCAGATGTTGACTAAAGGCTTGGTTGGTAGATATTCAGGAAAT GTTCACTGAATAAATAAGTAAATACATTATTGAAAAGCAAATCTGTATAAATGTG AAATTTTTATTTGTATTAGTAATAAAACATTAGTAGTTTA
1. How many bases are in the PAH gene DNA reference sequence?
79277 bp
2. How many bases are in the PAH gene mRNA reference sequence?
2680 bp
3. How many amino acids are in the PAH protein reference sequence?
452 aa
4. Briefly explain the differences in your answers to the first three questions. In other words, why does the DNA sequence have more bases than the mRNA sequence, and why does the mRNA sequence have more bases than the protein sequence has amino acids?
The DNA sequence has more bases because during transcription, DNA is cleaved and some portions are removed. The mRNA sequence has more bases than the protein sequence has amino acids because each amino acid is made up of multiple bases.
5. What is the base that is different between the normal PAH mRNA sequence and the mutant PAH mRNA sequence (base letters and position number)?
Position Number: 1369, Changes from G to A
1. 79,277 base pairs
2. 2,680 base pairs
3. 452 amino acids
4. The DNA sequence has more base pairs than the mRNA sequence because during transcription, the initial RNA transcrip is spliced in more than one way to produced mRNAs of varying lengths (all, though, less than the length of the original DNA molecule). The mRNA sequence has more bases than the protein sequence has amino acids because each amino acid is made up of multiple bases.
5. The base in the normal PAH mRNA sequence is Guanine, and the base in the mutant PAH mRNA sequence is Adenine at position number 1369.